Title

Phylogenetic occurrence of coiled coil proteins: implications for tissue structure in metazoa via a coiled coil tissue matrix

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

4-1-1996

Document Type

Article

Medical Subject Headings

Animals; Bacterial Proteins; Cells, Cultured; Cervix Uteri; Database Management Systems; Epithelial Cells; Epithelium; Female; Humans; *Phylogeny; Plant Proteins; Protein Conformation; Viral Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We examined GenBank sequence files with a heptad repeat analysis program to assess the phylogenetic occurrence of coiled coil proteins, how heptad repeat domains are organized within them, and what structural/functional categories they comprise. Of 102,007 proteins analyzed, 5.95% (6,074) contained coiled coil domains; 1.26% (1,289) contained "extended" (> 75 amino acid) domains. While the frequency of proteins containing coiled coils was surprisingly constant among all biota, extended coiled coil proteins were fourfold more frequent in the animal kingdom and may reflect early events in the divergence of plants and animals. Structure/function categories of extended coils also revealed phylogenetic differences. In pathogens and parasites, many extended coiled coil proteins are external and bind host proteins. In animals, the majority of extended coiled coil proteins were identified as constituents of two protein categories: 1) myosins and motors; or 2) components of the nuclear matrix-intermediate filament scaffold. This scaffold, produced by sequential extraction of epithelial monolayers in situ, contains only 1-2% of the cell mass while accurately retaining morphological features of living epithelium and is greatly enriched in proteins with extensive, interrupted coiled coil forming domains. The increased occurrence of this type of protein in metazoa compared with plants or protists leads us to hypothesize a tissue-wide matrix of coiled coil interactions underlying metazoan differentiated cell and tissue structure.

Rights and Permissions

Citation: Proteins. 1996 Apr;24(4):467-84. Link to article on publisher's site

Related Resources

Link to article in PubMed

PubMed ID

9162947