GSBS Student Publications

Title

ATP requirements and small interfering RNA structure in the RNA interference pathway

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

11-10-2001

Document Type

Article

Medical Subject Headings

Adenosine Triphosphate; Animals; Drosophila; *Gene Silencing; Models, Genetic; Phosphorylation; Proteins; *RNA Processing, Post-Transcriptional; RNA, Double-Stranded; RNA, Small Interfering; RNA, Untranslated

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We examined the role of ATP in the RNA interference (RNAi) pathway. Our data reveal two ATP-dependent steps and suggest that the RNAi reaction comprises at least four sequential steps: ATP-dependent processing of double-stranded RNA into small interfering RNAs (siRNAs), incorporation of siRNAs into an inactive approximately 360 kDa protein/RNA complex, ATP-dependent unwinding of the siRNA duplex to generate an active complex, and ATP-independent recognition and cleavage of the RNA target. Furthermore, ATP is used to maintain 5' phosphates on siRNAs. A 5' phosphate on the target-complementary strand of the siRNA duplex is required for siRNA function, suggesting that cells check the authenticity of siRNAs and license only bona fide siRNAs to direct target RNA destruction.

Rights and Permissions

Citation: Cell. 2001 Nov 2;107(3):309-21.

Related Resources

Link to article in PubMed

Journal Title

Cell

PubMed ID

11701122