GSBS Student Publications

Title

Spontaneous and cisplatin-induced recombination in Escherichia coli

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

6-5-2004

Document Type

Article

Medical Subject Headings

Cell Survival; Cisplatin; Cross-Linking Reagents; DNA Repair; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Gene Deletion; Gene Silencing; Genes, Bacterial; Lac Operon; Models, Genetic; Recombination, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

To measure cisplatin (cis-diaminodichloroplatinum(II))-induced recombination, we have used a qualitative intrachromosomal assay utilizing duplicate inactive lac operons containing non-overlapping deletions and selection for Lac+ recombinants. The two operons are separated by one Mb and conversion of one of them yields the Lac+ phenotype. Lac+ formation for both spontaneous and cisplatin-induced recombination requires the products of the recA, recBC, ruvA, ruvB, ruvC, priA and polA genes. Inactivation of the recF, recO, recR and recJ genes decreased cisplatin-induced, but not spontaneous, recombination. The dependence on PriA and RecBC suggests that recombination is induced following stalling or collapse of replication forks at DNA lesions to form double strand breaks. The lack of recombination induction by trans-DDP suggests that the recombinogenic lesions for cisplatin are purine-purine intrastrand crosslinks.

Rights and Permissions

Citation: DNA Repair (Amst). 2004 Jul 2;3(7):719-28. Link to article on publisher's site

DOI of Published Version

10.1016/j.dnarep.2004.02.009

Related Resources

Link to article in PubMed

Journal Title

DNA repair

PubMed ID

15177181