Signal transduction by the epidermal growth factor receptor after functional desensitization of the receptor tyrosine protein kinase activity
Graduate School of Biomedical Sciences; Howard Hughes Medical Institute; Program in Molecular Medicine
Medical Subject Headings
Adenosine Triphosphate; Amino Acid Sequence; Carcinoma, Squamous Cell; Cell Line; Epidermal Growth Factor; Humans; Inositol Phosphates; Kinetics; Molecular Sequence Data; Oligopeptides; Peptide Fragments; Phosphorylation; Protein-Tyrosine Kinases; Receptor, Epidermal Growth Factor; *Signal Transduction; Substrate Specificity; Tetradecanoylphorbol Acetate
Life Sciences | Medicine and Health Sciences
Previous work identified a protein kinase activity that phosphorylates the epidermal growth factor (EGF) receptor at Thr669. An assay for this protein kinase activity present in homogenates prepared from A431 human epidermoid carcinoma cells was developed using a synthetic peptide substrate corresponding to residues 663-681 of the EGF receptor (peptide T669). Here we report that a greater initial rate of T669 phosphorylation was observed in experiments using homogenates prepared from EGF- or phorbol ester-treated cells compared with control cells. EGF and 4 beta-phorbol 12-myristate 13-acetate (PMA) caused a 6-fold and a 2-fold increase in protein kinase activity, respectively. A kinetic analysis of T669 phosphorylation demonstrated that the increase in protein kinase activity observed was accounted for by an increase in Vmax. To examine the interaction between protein kinase C and signal transduction by the EGF receptor, the effect of pretreatment of cells with PMA on the subsequent response to EGF was investigated. Treatment of cells with PMA caused greater than 90% inhibition of the EGF-stimulated tyrosine phosphorylation of the EGF receptor and abolished the EGF-stimulated formation of soluble inositol phosphates. In contrast, PMA was not observed to inhibit the stimulation of T669 protein kinase activity caused by EGF. Thus, the apparent functional desensitization of the EGF receptor caused by PMA does not inhibit signal transduction mediated by the T669 protein kinase. Our results demonstrate that EGF receptor transmodulation alters the pattern of signal-transduction pathways that are utilized by the EGF receptor.
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Citation: Proc Natl Acad Sci U S A. 1990 Aug;87(16):6107-11.
Proceedings of the National Academy of Sciences of the United States of America
Northwood, Ingrid C. and Davis, Roger J., "Signal transduction by the epidermal growth factor receptor after functional desensitization of the receptor tyrosine protein kinase activity" (1990). GSBS Student Publications. 906.