GSBS Student Publications

Title

Complex T cell memory repertoires participate in recall responses at extremes of antigenic load

Student Author(s)

Shalyn C. Clute; Levi B. Watkin

GSBS Program

Immunology & Virology Program

UMMS Affiliation

Department of Pathology

Date

8-1-2006

Document Type

Article

Medical Subject Headings

Antigens, Viral; CD8-Positive T-Lymphocytes; Cell Adhesion; Cells, Cultured; Clone Cells; Dose-Response Relationship, Immunologic; Epitopes, T-Lymphocyte; Humans; *Immunologic Memory; Influenza A virus; Middle Aged; Peptide Fragments; Protein Binding; T-Lymphocyte Subsets; Viral Matrix Proteins

Disciplines

Immunology and Infectious Disease | Life Sciences | Medicine and Health Sciences

Abstract

The CD8 T cell memory response to the HLA-A2-restricted influenza epitope M1(58-66) can be an instructive model of immune memory to a nonevolving epitope of a frequently encountered pathogen that undergoes clearance. This memory repertoire can be complex, composed of a large number of clonotypes represented at low copy numbers, while maintaining a focus on the use of VB17 T cell receptors with identified Ag recognition motifs. Such a repertoire structure might provide a panoply of clonotypes whose differential avidity for the epitope would allow responses under varying antigenic loads. This possibility was tested experimentally by characterizing the responding repertoire in vitro while varying influenza Ag concentration over five orders of magnitude. At higher and lower Ag concentrations there was increased cell death, yet a focused but diverse response could still be observed. Thus, one of the characteristics of complex memory repertoires is to provide effector function at extremes of Ag load, a characteristic that is not generally considered in vaccination development but may be important in measuring its efficacy.

Rights and Permissions

Citation: J Immunol. 2006 Aug 1;177(3):2006-14.

Related Resources

Link to article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID

16849515