GSBS Student Publications

Title

Antiviral effect of lymphokine-activated killer cells: chemotaxis and homing to sites of virus infection

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology

Date

11-1-1989

Document Type

Article

Medical Subject Headings

Animals; Cells, Cultured; *Chemotaxis, Leukocyte; Interferon Type I; Interleukin-2; Killer Cells, Lymphokine-Activated; Mice; Mice, Inbred C57BL; Recombinant Proteins; Spleen; Vaccinia virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Lymphokine-activated killer (LAK) cells generated from C57BL/6 mouse spleen cells cultured with interleukin-2 are effective prophylactically against virus infection when inoculated at the site of virus injection. To predict the therapeutic efficacy of LAK cells, we determined whether LAK cells would home to sites of virus infection. In vitro, LAK cells responded chemotactically to cell-free peritoneal exudate fluids collected from virus-infected mice and to preparations of purified beta interferon. In vivo, radiolabeled LAK cells injected intravenously accumulated in the peritoneal cavities of intraperitoneally infected mice in amounts three to eight times greater than in uninfected mice. This ability to respond to chemotactic agents and migrate into sites of virus infection may make LAK cells useful as antiviral therapeutic agents.

Rights and Permissions

Citation: J Virol. 1989 Nov;63(11):4969-71.

Related Resources

Link to article in PubMed

Journal Title

Journal of virology

PubMed ID

2795722