Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Amino Acid Sequence; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Cell Division; Cell Nucleus; Cell Polarity; Cytoskeletal Proteins; Green Fluorescent Proteins; Intercellular Signaling Peptides and Proteins; Membrane Proteins; Molecular Sequence Data; Sequence Homology, Amino Acid; *Signal Transduction; Trans-Activators; Wnt Proteins; beta Catenin
Life Sciences | Medicine and Health Sciences
beta-Catenin regulates cell adhesion and cellular differentiation during development, and misregulation of beta-catenin contributes to numerous forms of cancer in humans. Here we describe Caenorhabditis elegans conditional alleles of mom-2/Wnt, mom-4/Tak1, and wrm-1/beta-catenin. We use these reagents to examine the regulation of WRM-1/beta-catenin during a Wnt-signaling-induced asymmetric cell division. While WRM-1 protein initially accumulates in the nuclei of all cells, signaling promotes the retention of WRM-1 in nuclei of responding cells. We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division.
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Citation: Genes Dev. 2005 Aug 1;19(15):1749-54. Link to article on publisher's site