GSBS Student Publications

Title

HiNF-P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology and Cancer Center

Date

12-14-2006

Document Type

Article

Medical Subject Headings

Animals; COS Cells; *Cell Cycle; Cell Cycle Proteins; Cell Line; Cell Line, Transformed; Cell Line, Tumor; Cell Transformation, Viral; Cercopithecus aethiops; Cyclin-Dependent Kinase Inhibitor p57; Genes, Reporter; Hela Cells; Histones; Humans; Luciferases; Nuclear Proteins; Promoter Regions (Genetics); Repressor Proteins; *Transcription, Genetic; Transfection

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Cell cycle progression beyond the G1/S phase transition requires the activation of a transcription complex containing histone nuclear factor P (HiNF-P) and nuclear protein mapped to ataxia telangiectasia (p220(NPAT)) in response to cyclin dependent kinase 2 (CDK2)/cyclin E signaling. We show here that the potent co-activating properties of HiNF-P/p220(NPAT) on the histone H4 gene promoter, which are evident in the majority of human cell types, are sporadically neutralized in distinct somatic cell lines. In cells where HiNF-P and p220(NPAT) do not activate the H4 gene promoter, HiNF-P instead represses transcription. Our data suggest that the cell type specific expression of the cyclin-dependent kinase inhibitory (CKI) protein p57(KIP2) inhibits the HiNF-P dependent activation of the histone H4 promoter. We propose that, analogous to E2F proteins and other cell cycle regulatory proteins, HiNF-P is a bifunctional transcriptional regulator that can activate or repress cell cycle controlled genes depending on the cellular context.

Rights and Permissions

Citation: J Cell Biochem. 2007 May 1;101(1):181-91. Link to article on publisher's site

DOI of Published Version

10.1002/jcb.21157

Related Resources

Link to article in PubMed

Journal Title

Journal of cellular biochemistry

PubMed ID

17163457