GSBS Student Publications

Title

Poly(lactide-co-glycolide) microspheres as a moldable scaffold for cartilage tissue engineering

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

12-4-2004

Document Type

Article

Medical Subject Headings

*Absorbable Implants; Animals; Biocompatible Materials; Cartilage, Articular; Cattle; Cell Adhesion; Cell Culture Techniques; Cell Differentiation; Cell Proliferation; Cells, Cultured; Chondrocytes; Feasibility Studies; Lactic Acid; Materials Testing; Microspheres; Polyglycolic Acid; Polymers; Porosity; Surface Properties; Tissue Engineering

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

This study demonstrates the use of biodegradable poly(lactide-co-glycolide) (PLG) microspheres as a moldable scaffold for cartilage tissue engineering. Chondrocytes were delivered to a cylindrical mold with or without PLG microspheres and cultured in vitro for up to 8 weeks. Cartilagenous tissue formed using chondrocytes and microspheres maintained thickness, shape, and chondrocyte collagen type II phenotype, as indicated by type II collagen staining. The presence of microspheres further enhanced total tissue mass and the amount of glycosaminoglycan that accumulated. Evaluation of microsphere composition demonstrated effects of polymer molecular weight, end group chemistry, and buffer inclusion on tissue-engineered cartilage growth. Higher molecular weight PLG resulted in a larger mass of cartilage-like tissue formed and a higher content of proteoglycans. Cartilage-like tissue formed using microspheres made from low molecular weight and free carboxylic acid end groups did not display increases in tissue mass, yet a modest increased proteoglycan accumulation was detected. Microspheres comprised of PLG with methyl ester end groups yielded a steady increase in tissue mass, with no real increase in matrix accumulation. The microencapsulation of Mg(OH)(2) had negative effects on tissue mass and matrix accumulation. The data herein reflect the potential utility of a moldable PLG-chondrocyte system for tissue-engineering applications.

Rights and Permissions

Citation: Biomaterials. 2005 May;26(14):1945-52. Link to article on publisher's site

DOI of Published Version

10.1016/j.biomaterials.2004.06.030

Related Resources

Link to article in PubMed

Journal Title

Biomaterials

PubMed ID

15576168