GSBS Student Publications

Title

A novel injectable approach for cartilage formation in vivo using PLG microspheres

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

4-21-2004

Document Type

Article

Medical Subject Headings

Animals; Biocompatible Materials; Cartilage, Articular; Cattle; Cell Division; Cell Survival; Cell Transplantation; Cells, Cultured; Chondrocytes; Feasibility Studies; Glycolates; Glycosaminoglycans; Injections; Materials Testing; Mice; Microspheres; Tissue Engineering

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

This study documents the use of biodegradable poly(lactide-co-glycolide) (PLG) microspheres as a novel, injectable scaffold for cartilage tissue engineering. Chondrocytes were delivered via injection to the subcutaneous space of athymic mice in the presence and absence of PLG microspheres. Tissue formation was evaluated up to 8 weeks post-injection. Progressive cartilage formation was observed in samples containing microspheres. The presence of microspheres increased the quantity of tissue formed, the amount of glycosaminoglycan that accumulated, and the uniformity of type II collagen deposition. Microsphere composition influenced the growth of the tissue engineered cartilage. Higher molecular weight PLG resulted in a larger mass of cartilage formed and a higher content of proteoglycans. Microspheres comprised PLG with methyl ester end groups yielded increased tissue mass and matrix accumulation, but did not display homogenous matrix deposition. The microencapsulation of Mg(OH)2 had negative effects on tissue mass and matrix accumulation. Matrix accumulation, cell number, and tissue mass were unchanged by microsphere size, but larger microspheres increased the frequency of central necrosis in implants. The data herein reflect the promising utility of an injectable PLG-chondrocyte system for tissue engineering applications.

Rights and Permissions

Citation: Ann Biomed Eng. 2004 Mar;32(3):418-29.

Related Resources

Link to article in PubMed

Journal Title

Annals of biomedical engineering

PubMed ID

15095816