GSBS Student Publications

Title

Synapsin I gene expression in the adult rat brain with comparative analysis of mRNA and protein in the hippocampus

Student Author(s)

Richard H. Melloni

GSBS Program

Cell Biology

UMMS Affiliation

Department of Neurology; Department of Cell Biology; Department of Anesthesiology

Date

1-22-1993

Document Type

Article

Medical Subject Headings

Animals; Brain; Brain Mapping; Gene Expression Regulation; Hippocampus; In Situ Hybridization; Male; Nerve Tissue Proteins; RNA, Messenger; Rats; Rats, Sprague-Dawley; Synapsins

Disciplines

Cellular and Molecular Physiology | Molecular and Cellular Neuroscience | Neurology

Abstract

Synapsin I is the best characterized member of a family of neuron-specific phosphoproteins thought to be involved in the regulation of neurotransmitter release. In this report, we present the first extensive in situ hybridization study detailing the regional and cellular distribution of synapsin I mRNA in the adult rat brain. Both the regional distribution and relative levels of synapsin I mRNA established by in situ hybridization were confirmed by RNA blot analysis. Our data demonstrate the widespread yet regionally variable expression of synapsin I mRNA throughout the adult rat brain. The greatest abundance of synapsin I mRNA was found in the pyramidal neurons of the CA3 and CA4 fields of the hippocampus, and in the mitral and internal granular cell layers of the olfactory bulb. Other areas abundant in synapsin I mRNA were the layer II neurons of the piriform cortex and layer II and V neurons of the entorhinal cortex, the granule cell neurons of the dentate gyrus, the pyramidal neurons of hippocampal fields CA1 and CA2, and the cells of the parasubiculum. In general, the pattern of expression of synapsin I mRNA paralleled those encoding other synaptic terminal-specific proteins, such as synaptophysin, VAMP-2, and SNAP-25, with noteworthy exceptions. To determine specifically how synapsin I mRNA levels are related to levels of synapsin I protein, we examined in detail the local distribution patterns of both synapsin I mRNA and protein in the rat hippocampus. These data revealed differential levels of expression of synapsin I mRNA and protein within defined synaptic circuits of the rat hippocampus.

Rights and Permissions

Citation: J Comp Neurol. 1993 Jan 22;327(4):507-20. Link to article on publisher's site

DOI of Published Version

10.1002/cne.903270404

Related Resources

Link to article in PubMed

Journal Title

The Journal of comparative neurology

PubMed ID

8440778