GSBS Student Publications

Title

A method for the direct measurement of mRNA in discrete regions of mammalian brain

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Neurology

Date

1-11-1992

Document Type

Article

Medical Subject Headings

Animals; Autoradiography; *Brain Chemistry; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Liver; Male; Nucleic Acid Hybridization; RNA, Messenger; RNA, Ribosomal; Rats; Rats, Inbred Strains

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

A rapid and nearly quantitative method for the direct analysis of steady-state mRNA levels in microgram quantities of frozen mammalian brain is described. Briefly, tissue punches 0.5-1.0 mm in diameter were sampled from 250-microns-thick cryostat sections of rat brain (approximately 50-200 micrograms tissue). The samples were homogenized in 50 microliters of a denaturing gel loading buffer and applied directly to a 2.2 M formaldehyde-agarose gel for electrophoresis and subsequent RNA blot analysis. The method is extremely rapid, results in excellent recovery of intact RNA, and allows the direct assay of mRNA levels in discrete subregions of the mammalian brain.

Rights and Permissions

Citation: Anal Biochem. 1992 Jan;200(1):95-9.

Related Resources

Link to article in PubMed

Journal Title

Analytical biochemistry

PubMed ID

1595906