GSBS Student Publications

Title

Expression of cell growth and bone phenotypic genes during the cell cycle of normal diploid osteoblasts and osteosarcoma cells

UMMS Affiliation

Department of Cell Biology

Date

10-1-1994

Document Type

Article

Medical Subject Headings

Actins; Alkaline Phosphatase; Animals; Bone Neoplasms; Bone and Bones; Cell Cycle; Cell Division; Cell Line; Cells, Cultured; Collagen; Diploidy; Extracellular Matrix; Fetus; *Gene Expression; Histones; Osteoblasts; Osteocalcin; Osteopontin; Osteosarcoma; Phenotype; Rats; Sialoglycoproteins; Skull; Tumor Cells, Cultured; Vimentin

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Establishing regulatory mechanisms that mediate proliferation of osteoblasts while restricting expression of genes associated with mature bone cell phenotypic properties to post-proliferative cells is fundamental to understanding skeletal development. To gain insight into relationships between growth control and the developmental expression of genes during osteoblast differentiation, we have examined expression of three classes of genes during the cell cycle of normal diploid rat calvarial-derived osteoblasts and rat osteosarcoma cells (ROS 17/2.8): cell cycle and growth-related genes (e.g., histone), genes that encode major structural proteins (e.g., actin and vimentin), and genes related to the biosynthesis, organization, and mineralization of the bone extracellular matrix (e.g., alkaline phosphatase, collagen I, osteocalcin, and osteopontin). In normal diploid osteoblasts as well as in osteosarcoma cells we found that histone genes, required for cell progression, are selectively expressed during S phase. All other genes studied were constitutively expressed both at the transcriptional and posttranscriptional levels. Alkaline phosphatase, an integral membrane protein in both osteoblasts and osteosarcoma cells, exhibited only minimal changes in activity during the osteoblast and osteosarcoma cell cycles. Our findings clearly indicate that despite the loss of normal proliferation-differentiation interrelationships in osteosarcoma cells, cell cycle regulation or constitutive expression of growth and phenotypic genes is maintained.

Rights and Permissions

Citation: J Cell Biochem. 1994 Oct;56(2):274-82. doi #10.1002/jcb.240560221

Related Resources

Link to article in PubMed

Journal Title

Journal of cellular biochemistry

PubMed ID

7829587