Wingless signaling at synapses is through cleavage and nuclear import of receptor DFrizzled2
Department of Neurobiology
Medical Subject Headings
Active Transport, Cell Nucleus; Amino Acid Sequence; Animals; Cell Line; Cell Nucleus; Drosophila Proteins; Drosophila melanogaster; Endocytosis; Frizzled Receptors; Molecular Sequence Data; Muscle Cells; Mutagenesis, Site-Directed; Neuromuscular Junction; Protein Binding; Proto-Oncogene Proteins; Receptors, G-Protein-Coupled; Receptors, Neurotransmitter; *Signal Transduction; Synaptic Membranes; Transfection; Transgenes
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Wingless secretion provides pivotal signals during development by activating transcription of target genes. At Drosophila synapses, Wingless is secreted from presynaptic terminals and is required for synaptic growth and differentiation. Wingless binds the seven-pass transmembrane DFrizzled2 receptor, but the ensuing events at synapses are not known. We show that DFrizzled2 is endocytosed from the postsynaptic membrane and transported to the nucleus. The C terminus of DFrizzled2 is cleaved and translocated into the nucleus; the N-terminal region remains just outside the nucleus. Translocation of DFrizzled2-C into the nucleus, but not its cleavage and transport, depends on Wingless signaling. We conclude that, at synapses, Wingless signal transduction occurs through the nuclear localization of DFrizzled2-C for potential transcriptional regulation of synapse development.
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Citation: Science. 2005 Nov 25;310(5752):1344-7. Link to article on publisher's site