GSBS Student Publications

Title

Identification of murine poxvirus-specific CD8+ CTL epitopes with distinct functional profiles

UMMS Affiliation

Center for Infectious Disease and Vaccine Research

Date

2-9-2005

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Cell Degranulation; Cell Line, Tumor; Cells, Cultured; *Cytotoxicity Tests, Immunologic; Epitopes, T-Lymphocyte; H-2 Antigens; Injections, Intraperitoneal; Interferon Type II; Kinetics; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Peptide Fragments; T-Lymphocytes, Cytotoxic; Vaccinia virus; Viral Vaccines

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Murine T cell epitopes against vaccinia virus (VV) have not been characterized to date in part due to the large and complex genome of VV. We have identified and characterized two CD8+ T cell epitopes on the A47L (modified VV Ankara strain (MVA)-029) and J6R (MVA-043) proteins of VV that are Db and Kb restricted, respectively. Following i.p. immunization with VV New York City Board of Health (NYCBH) strain, MVA-029 peptide-stimulated splenocytes secreted IFN-gamma from 7 days to 7 mo postimmunization, and virus-stimulated effectors were also able to lyse MVA-029-pulsed target cells at the same time points. In contrast, MVA-043 peptide-stimulated splenocytes secreted very low levels of IFN-gamma only at day 7 but maintained the ability to lyse target cells up to 2 mo postimmunization. Both MVA-029 and MVA-043 peptide-stimulated lymph node cells degranulated similarly as assessed by Ag-induced CD107 expression. T cell responses to whole-virus stimulation remained robust and steady during the acute and memory T cell response to VV. Identification of T cell epitopes on VV will enable further studies to increase our understanding of the role of CD8+ T cells in VV infection and assist in the design of new protective strategies.

Rights and Permissions

Citation: J Immunol. 2005 Feb 15;174(4):2212-9.

Related Resources

Link to article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID

15699154