Impaired T cell proliferation in acute dengue infection
Graduate School of Biomedical Sciences; Center for Infectious Disease and Vaccine Research
Medical Subject Headings
Acute Disease; Antibodies, Monoclonal; Antigen-Presenting Cells; Antigens, CD28; Antigens, Viral; Cell Communication; Child; Dengue; Dengue Hemorrhagic Fever; Dengue Virus; Gamma Rays; Humans; Immune Tolerance; Interleukin-12; Interleukin-2; Interleukin-4; Interleukin-7; Leukocyte Count; Leukocytes, Mononuclear; Lymphocyte Activation; Lymphocyte Count; Monocytes; Phytohemagglutinins; Recombinant Proteins; T-Lymphocytes; Tetanus Toxoid
Life Sciences | Medicine and Health Sciences
Decreased proliferative responses to mitogens and recall Ags have been observed in PBMC obtained during several acute human viral infections. To determine whether cell-mediated responses are altered during acute dengue infection, we examined the proliferative responses of PBMC from children enrolled in a prospective study of dengue infections in Thailand. All responses of PBMC during acute illness were compared with the same patients' PBMC obtained at least 6 mo after their infection. Proliferative responses to PHA, anti-CD3, tetanus toxoid, and dengue Ags were decreased significantly in PBMC obtained during the acute infection. The proliferative responses to PHA were restored by the addition of gamma-irradiated autologous convalescent or allogeneic PBMC. Cell contact with the irradiated PBMC was necessary to restore proliferation. Non-T cells from the acute PBMC of dengue patients did not support proliferation of T cells from control donors in response to PHA, but T cells from the PBMC of patients with acute dengue proliferated if accessory cells from a control donor were present. Addition of anti-CD28 Abs restored anti-CD3-induced proliferation of the PBMC of some patients. The percentage of monocytes was reduced in the acute sample of PBMC of the dengue patients. Addition of IL-2 or IL-7, but not IL-4 or IL-12, also restored proliferation of acute PBMC stimulated with anti-CD3. The results demonstrate that both quantitative and qualitative defects in the accessory cell population during acute dengue illness result in a depression of in vitro T cell proliferation.
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Citation: J Immunol. 1999 May 1;162(9):5609-15.
Journal of immunology (Baltimore, Md. : 1950)
Mathew, Anuja; Kurane, Ichiro; Green, Sharone; Vaughn, David W.; Kalayanarooj, Siripen; Suntayakorn, Saroj; Ennis, Francis A.; and Rothman, Alan L., "Impaired T cell proliferation in acute dengue infection" (1999). GSBS Student Publications. 817.