GSBS Student Publications

Title

Predominance of HLA-restricted cytotoxic T-lymphocyte responses to serotype-cross-reactive epitopes on nonstructural proteins following natural secondary dengue virus infection

UMMS Affiliation

Graduate School of Biomedical Sciences; Center for Infectious Disease and Vaccine Research

Date

4-29-1998

Document Type

Article

Medical Subject Headings

CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cells, Cultured; Child; Cross Reactions; Dengue; Dengue Virus; Epitope Mapping; Epitopes, T-Lymphocyte; HLA Antigens; Humans; Leukocytes, Mononuclear; RNA Helicases; Serine Endopeptidases; Serotyping; T-Lymphocytes, Cytotoxic; Thailand; Viral Nonstructural Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We examined the memory cytotoxic T-lymphocytic (CTL) responses of peripheral blood mononuclear cells (PBMC) obtained from patients in Thailand 12 months after natural symptomatic secondary dengue virus infection. In all four patients analyzed, CTLs were detected in bulk culture PBMC against nonstructural dengue virus proteins. Numerous CD4+ and CD8+ CTL lines were generated from the bulk cultures of two patients, KPP94-037 and KPP94-024, which were specific for NS1.2a (NS1 and NS2a collectively) and NS3 proteins, respectively. All CTL lines derived from both patients were cross-reactive with other serotypes of dengue virus. The CD8+ NS1.2a-specific lines from patient KPP94-037 were HLA B57 restricted, and the CD8+ NS3-specific lines from patient KPP94-024 were HLA B7 restricted. The CD4+ CTL lines from patient KPP94-037 were HLA DR7 restricted. A majority of the CD8+ CTLs isolated from patient KPP94-024 were found to recognize amino acids 221 to 232 on NS3. These results demonstrate that in Thai patients after symptomatic secondary natural dengue infections, CTLs are mainly directed against nonstructural proteins and are broadly cross-reactive.

Rights and Permissions

Citation: J Virol. 1998 May;72(5):3999-4004.

Related Resources

Link to article in PubMed

Journal Title

Journal of virology

PubMed ID

9557687