Transient decreases in human T cell proliferative responses following vaccinia immunization
Graduate School of Biomedical Sciences; Center for Infectious Disease and Vaccine Research
Medical Subject Headings
Antibodies; Antigen-Presenting Cells; Antigens, CD28; Antigens, CD3; Antigens, Viral; Humans; Immunization; Interleukin-2; Leukocytes, Mononuclear; Lymphocyte Activation; Recombinant Proteins; T-Lymphocytes; Vaccinia virus
Life Sciences | Medicine and Health Sciences
To further study the immunosuppression associated with virus infections, we analyzed the proliferative responses of serial PBMC samples obtained following vaccinia virus immunization. In four of five volunteers, responses to PHA, anti-CD3, vaccinia virus, and recall antigens were markedly decreased at at least one time point between days 5 and 29 after vaccination. Responses to PHA were restored by the addition of IL-2 or irradiated autologous healthy PBMC in the two volunteers tested, suggesting that the proliferation defect is attributable to accessory cell dysfunction. In one donor, immobilized anti-CD3 failed to induce proliferation, but addition of immobilized anti-CD28 partially restored proliferation. These results indicate that vaccinia virus infection can transiently suppress proliferative responses of PBMC, in part by causing accessory cell dysfunction. Our findings extend the list of viral infections associated with systemic immunologic effects and demonstrate that suppression of proliferation can occur with localized virus infections.
Rights and Permissions
Citation: Clin Immunol. 2000 Aug;96(2):100-7. Link to article on publisher's site
DOI of Published Version
Clinical immunology (Orlando, Fla.)
Mathew, Anuja; Ennis, Francis A.; and Rothman, Alan L., "Transient decreases in human T cell proliferative responses following vaccinia immunization" (2000). GSBS Student Publications. 815.