GSBS Student Publications

Title

Meiosis in Saccharomyces cerevisiae mutants lacking the centromere-binding protein CP1

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

5-1-1992

Document Type

Article

Medical Subject Headings

Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Chromosomes, Fungal; DNA-Binding Proteins; Fungal Proteins; Genes, Fungal; Meiosis; Mitosis; Plasmids; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Spores, Fungal

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

CP1 (encoded by the CEP1 gene) is a centromere binding protein of Saccharomyces cerevisiae that binds to the conserved DNA element I (CDEI) of yeast centromeres. To investigate the function of CP1 in yeast meiosis, we analyzed the meiotic segregation of CEN plasmids, nonessential chromosome fragments (CFs) and chromosomes in cep1 null mutants. Plasmids and CFs missegregated in 10-20% of meioses with the most frequent type of aberrant event being precocious sister segregation at the first meiotic division; paired and unpaired CFs behaved similarly. An unpaired chromosome I homolog (2N + 1) also missegregated at high frequency in the cep1 mutant (7.6%); however, missegregation of other chromosomes was not detected by tetrad analysis. Spore viability of cep1 tetrads was significantly reduced, and the pattern of spore death was nonrandom. The inviability could not be explained solely by chromosome missegregation and is probably a pleiotropic effect of cep1. Mitotic chromosome loss in cep1 strains was also analyzed. Both simple loss (1:0 segregation) and nondisjunction (2:0 segregation) were increased, but the majority of loss events resulted from nondisjunction. We interpret the results to suggest that CP1 generally promotes chromatid-kinetochore adhesion.

Rights and Permissions

Citation: Genetics. 1992 May;131(1):43-53.

Related Resources

Link to article in PubMed

Journal Title

Genetics

PubMed ID

1592241