GSBS Student Publications

Title

myc and E1A oncogenes alter the responses of PC12 cells to nerve growth factor and block differentiation

UMMS Affiliation

Graduate School of Biomedical Sciences; Center for Cancer Research

Date

1-1-1987

Document Type

Article

Medical Subject Headings

Adenovirus Early Proteins; *Cell Differentiation; *Cell Division; DNA Replication; Enzyme Induction; Nerve Growth Factors; Oncogene Proteins, Viral; *Oncogenes; Ornithine Decarboxylase; Pheochromocytoma; Receptors, Cell Surface; Receptors, Nerve Growth Factor; Tumor Cells, Cultured

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

PC12 rat pheochromocytoma cells respond to nerve growth factor (NGF) by neuronal differentiation and partial growth arrest. Mouse c-myc and adenovirus E1A genes were introduced into PC12 cells to study the influence of these nuclear oncogenes on neuronal differentiation. Expression of myc or E1A blocked morphological differentiation and caused NGF to stimulate rather than inhibit cell proliferation. NGF binding to cell surface receptors and ornithine decarboxylase induction were similar in myc- and E1A-expressing clones compared with wild-type PC12 cells, suggesting that changes in the cellular response to NGF were at a post-receptor level. These results illustrate that NGF can promote either growth or differentiation of PC12 cells, and that myc or E1A alter the phenotypic responses to growth factors and hormones.

Rights and Permissions

Citation: Oncogene. 1987;1(4):361-7.

Related Resources

Link to article in PubMed

Journal Title

Oncogene

PubMed ID

2838784

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