GSBS Student Publications

Title

The skeletal effects of colony-stimulating factor-1 in toothless (osteopetrotic) rats: persistent metaphyseal sclerosis and the failure to restore subepiphyseal osteoclasts

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

7-1-1993

Document Type

Article

Medical Subject Headings

Animals; Bone Regeneration; Bone and Bones; Macrophage Colony-Stimulating Factor; Osteoblasts; Osteoclasts; Osteopetrosis; Rats; Rats, Mutant Strains; Sclerosis; Time Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Toothless (tl), one of four osteopetrotic mutations in the rat, is characterized by few osteoclasts, undetectable bone resorption, and failure of correction by bone marrow transplantation. We recently reported that CSF-1 treatment improves these skeletal problems but that metaphyseal sclerosis persists. In the present study we demonstrate that optimal reduction of the skeletal sclerosis in tl rats following CSF-1 treatment has lower and upper dosage thresholds and that skeletal sclerosis returns after CSF-1 withdrawal. Osteoclasts increase significantly in tl rats after CSF-1 treatment, but compared to untreated normal littermates, histochemical staining for characteristic enzymes and osteoclast number is reduced and no osteoclasts appear in the subepiphyseal areas of long bones. These data are interpreted to mean that there are dosage limits to the beneficial skeletal effects of CSF-1, that persistent sclerosis is related to the failure to restore subepiphyseal osteoclasts, that osteoclast or progenitor populations may be deficient or differ in their responses to CSF-1, and that the defect in tl rats is not merely lack of a circulating, biologically active form of CSF-1.

Rights and Permissions

Citation: Bone. 1993 Jul-Aug;14(4):675-80.

Related Resources

Link to article in PubMed

Journal Title

Bone

PubMed ID

8274312