B lymphocyte development and activation independent of MHC class II expression
UMass Chan Affiliations
Department of Cancer BiologyProgram in Immunology and Virology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1993-02-15Keywords
Animals; Antibody Formation; B-Lymphocytes; Cell Differentiation; Flow Cytometry; *Genes, MHC Class II; Histocompatibility Antigens Class II; Leukocyte Count; *Lymphocyte Activation; Lymphocyte Cooperation; Mice; Mutagenesis, Insertional; Receptors, Antigen, B-Cell; T-LymphocytesLife Sciences
Medicine and Health Sciences
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A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgG1 (and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II-animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.Source
J Immunol. 1993 Feb 15;150(4):1223-33.
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http://hdl.handle.net/20.500.14038/34148PubMed ID
8432977Related Resources
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