Regulation of mRNA stability and the poly(A) problem in Dictyostelium discoideum
Authors
Manrow, Richard E.Shapiro, Robert A.
Herrick, David
Steel, Laura F.
Blinder, Dmitry B.
Jacobson, Allan
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1988-01-01Keywords
Dictyostelium; Poly A; RNA; *RNA Processing, Post-Transcriptional; RNA, MessengerLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
This paper reviews our studies of three aspects of post-transcriptional regulation in Dictyostelium discoideum: 1) the determinants of mRNA stability in vegetative amoebae; 2) the effects of disaggregation and cyclic AMP on the decay rates of cell-type-specific mRNAs in late developing cells; and 3) the cytoplasmic function of the 3' poly(A) tracts present on most mRNAs. We find that: 1) mRNA stability in vegetative amoebae is not dependent on mRNA size, ribosome loading, or poly(A) tract length, but may be determined by specific 3'-untranslated sequences within a given mRNA; 2) mRNA decay rates in late developing cells are heterogeneous, and cyclic AMP does not act directly to stabilize cell-type-specific mRNAs; and 3) poly(A) is most likely involved in the initiation of protein synthesis via an interaction with cytoplasmic poly(A)-binding proteins.Source
Dev Genet. 1988;9(4-5):403-19. Link to article on publisher's siteDOI
10.1002/dvg.1020090420Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34144PubMed ID
2468435Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/dvg.1020090420