GSBS Student Publications

Title

IL-16 represses HIV-1 promoter activity

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Virology and Immunology; Department of Medicine, Division of Pulmonary, Allergy & Critical Care

Date

1-1-1997

Document Type

Article

Medical Subject Headings

Down-Regulation; Gene Products, tat; HIV Long Terminal Repeat; HIV-1; Humans; Interleukin-16; NF-kappa B; Virus Activation; tat Gene Products, Human Immunodeficiency Virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

IL-16 is produced by CD8+ lymphocytes and has been reported to inhibit HIV-1 and SIV replication in infected PBMCs. CD4 serves as a receptor for the secreted form of IL-16, and IL-16 binding to CD4 induces signal transduction, which affects the activation state of the cell. We hypothesized, therefore, that the effect of IL-16 on HIV-1 replication might occur at the level of virus expression. In transient transfection studies with HIV-1 LTR-reporter gene constructs we found that pretreatment of CD4+ lymphoid cells with recombinant IL-16 repressed HIV-1 promoter activity up to 60-fold, preventing both PMA and Tat activation. This effect of IL-16 required sequences contained within the core enhancer, but was not simply due to the down-regulation of transcription factors binding to this element.

Rights and Permissions

Citation: J Immunol. 1997 Jan 1;158(1):5-8.

Related Resources

Link to article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID

8977168