IL-16 represses HIV-1 promoter activity
Graduate School of Biomedical Sciences; Program in Virology and Immunology; Department of Medicine, Division of Pulmonary, Allergy & Critical Care
Medical Subject Headings
Down-Regulation; Gene Products, tat; HIV Long Terminal Repeat; HIV-1; Humans; Interleukin-16; NF-kappa B; Virus Activation; tat Gene Products, Human Immunodeficiency Virus
Life Sciences | Medicine and Health Sciences
IL-16 is produced by CD8+ lymphocytes and has been reported to inhibit HIV-1 and SIV replication in infected PBMCs. CD4 serves as a receptor for the secreted form of IL-16, and IL-16 binding to CD4 induces signal transduction, which affects the activation state of the cell. We hypothesized, therefore, that the effect of IL-16 on HIV-1 replication might occur at the level of virus expression. In transient transfection studies with HIV-1 LTR-reporter gene constructs we found that pretreatment of CD4+ lymphoid cells with recombinant IL-16 repressed HIV-1 promoter activity up to 60-fold, preventing both PMA and Tat activation. This effect of IL-16 required sequences contained within the core enhancer, but was not simply due to the down-regulation of transcription factors binding to this element.
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Citation: J Immunol. 1997 Jan 1;158(1):5-8.
Journal of immunology (Baltimore, Md. : 1950)
Maciaszek, Joseph Walter; Parada, Nereida A.; Cruikshank, William W.; Center, David M.; Kornfeld, Hardy; and Viglianti, Gregory A., "IL-16 represses HIV-1 promoter activity" (1997). GSBS Student Publications. 799.