GSBS Student Publications

Title

Gene transfer to human cells using retrovirus vectors produced by a new polytropic packaging cell line

UMMS Affiliation

Graduate School of Biomedical Sciences; Graduate Program in Immunology and Virology

Date

6-1-1997

Document Type

Article

Medical Subject Headings

3T3 Cells; Animals; CHO Cells; *Cell Line; Cricetinae; Defective Viruses; *Gene Transfer Techniques; *Genetic Vectors; Humans; Hybrid Cells; Mice; Mink Cell Focus-Inducing Viruses; Muridae; Retroviridae; Species Specificity; Viral Interference; Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We report here the construction of a new packaging cell line, called MPAC, that packages defective retroviral vectors in viral particles with envelope proteins derived from a Moloney mink cell focus-inducing (MCF) polytropic virus. We characterized the tropism of MPAC-packaged retroviral vectors and show that some human cell lines can be infected with these vectors while others cannot. In addition, we show that some human cells fully support MCF virus replication while others either partially or fully restrict MCF virus replication.

Rights and Permissions

Citation: J Virol. 1997 Jun;71(6):4825-8.

Related Resources

Link to article in PubMed

Journal Title

Journal of virology

PubMed ID

9151879