T-lymphocyte downregulation after acute viral infection is not dependent on CD95 (Fas) receptor-ligand interactions
Graduate School of Biomedical Sciences; Department of Pathology
Medical Subject Headings
Acute Disease; Animals; Antigens, CD95; Apoptosis; Down-Regulation; Fas Ligand Protein; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Male; Membrane Glycoproteins; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred MRL lpr; Spleen; T-Lymphocytes
Life Sciences | Medicine and Health Sciences
Infection of mice with lymphocytic choriomeningitis virus (LCMV) causes a major expansion of CD8+ T cells followed by a period of immune downregulation that coincides with the induction of lymphocyte apoptosis in the mouse spleen. CD95 (Fas) and its ligand are important for regulating peripheral T-lymphocyte numbers and can mediate apoptosis of mature T lymphocytes. We infected CD95- and CD95L-deficient mice (lpr and gld, respectively) with LCMV to determine if the immune downregulation that occurred following resolution of the LCMV infection was due to a CD95-dependent apoptotic mechanism. Lymphocytes from LCMV-infected lpr and gld mice were capable of normal T-cell expansion and cytolytic function but were, in contrast to activated cells from normal virus-infected mice, relatively more resistant to T-cell receptor-induced apoptosis in vitro. However, in vivo there were significant numbers of apoptotic cells in the spleens of lpr and gld mice recovering from the infection, and the T-cell number and cytolytic activity decreased to normal postinfection levels. Thus, CD95 is not required for the immune downregulation of the CD8+-T-lymphocyte response following acute LCMV infection.
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Citation: J Virol. 1996 Nov;70(11):8199-203.
Journal of virology
Lohman, Barbara L.; Razvi, Enal Shahid; and Welsh, Raymond M., "T-lymphocyte downregulation after acute viral infection is not dependent on CD95 (Fas) receptor-ligand interactions" (1996). GSBS Student Publications. 780.