Simian immunodeficiency virus-specific cytotoxic T-lymphocyte induction through DNA vaccination of rhesus monkeys
Graduate School of Biomedical Sciences; Department of Medicine; Department of Pathology
Medical Subject Headings
Animals; DNA, Viral; Gene Products, env; Histocompatibility Antigens Class I; Macaca mulatta; Plasmids; Retroviridae Proteins; Simian immunodeficiency virus; T-Lymphocytes, Cytotoxic; Tumor Cells, Cultured; Vaccines, Synthetic; Viral Envelope Proteins; Viral Vaccines
Life Sciences | Medicine and Health Sciences
In view of the growing evidence that virus-specific cytotoxic T lymphocytes (CTL) play an important role in containing the early spread of human immunodeficiency virus type 1 (HIV-1) in infected individuals, novel vaccine strategies capable of eliciting HIV-1-specific CTL are being pursued in attempts to create an effective AIDS vaccine. We have used the simian immunodeficiency virus of macaques (SIVmac)/rhesus monkey model to explore the induction of AIDS virus-specific CTL responses by DNA vaccination. We found that the inoculation of rhesus monkeys with plasmid DNA encoding SIVmac Env and Gag elicited a persisting SIVmac-specific memory CTL response. These CTL were CD8+ and major histocompatibility complex class I restricted. These studies provide evidence for the potential utility of DNA inoculation as an approach to an HIV-1 vaccine.
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Citation: J Virol. 1996 Jan;70(1):678-81.
Journal of virology
Yasutomi, Yasuhiro; Robinson, Harriet L.; Lu, Shan; Mustafa, Farah; Lekutis, Christine; Arthos, James; Mullins, James I.; Voss, Gerald; Manson, Kelledy; Wyand, Michael; and Letvin, Norman L., "Simian immunodeficiency virus-specific cytotoxic T-lymphocyte induction through DNA vaccination of rhesus monkeys" (1996). GSBS Student Publications. 754.