GSBS Student Publications

Title

Early studies on DNA-based immunizations for measles virus

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology

Date

6-1-1997

Document Type

Article

Medical Subject Headings

Animals; Antibodies, Viral; Biolistics; Cercopithecus aethiops; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Measles Vaccine; Measles virus; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Rabbits; Vaccines, DNA; Vero Cells

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

DNA-mediated immunizations have been used to raise neutralizing antibodies for measles virus. Single inoculations of plasmids expressing measles hemagglutinin or fusion glycoproteins raised neutralizing antibody in BALB/c mice. Plasmids expressing the hemagglutinin glycoprotein (both normal and secreted) raised neutralizing responses that persisted for 1 year. For both forms of hemagglutinin, the effectiveness of the raised antibody (ratio of neutralizing activity to ELISA activity) was similar. High titers of neutralizing antibody were also raised by inoculation of rabbits with the hemagglutinin and fusion glycoprotein-expressing plasmids.

Rights and Permissions

Citation: Vaccine. 1997 Jun;15(8):888-91.

Related Resources

Link to Article in PubMed

Journal Title

Vaccine

PubMed ID

9234540