Coexpression of B7-1 and antigen blocks tolerance induction to antigen presented by resting B cells
Department of Molecular Genetics and Microbiology; Graduate School of Biomedical Sciences
Medical Subject Headings
Animals; *Antigen Presentation; Antigen-Presenting Cells; Antigens, CD80; B-Lymphocytes; Female; Humans; Immunization, Secondary; Immunoglobulin mu-Chains; Injections, Intravenous; Interphase; Lipopolysaccharides; *Lymphocyte Activation; Lymphocyte Transfusion; Male; Mice; Mice, Transgenic; *Self Tolerance
Life Sciences | Medicine and Health Sciences
To investigate a role for B cells as tolerogenic APCs in peripheral lymphoid organs, we have developed a system in which B cells from mice transgenic for the membrane-bound form of human mu-chain are transferred into nontransgenic recipients. Mice injected with B cells expressing human mu-chain became profoundly tolerant to human mu-chain, as shown by greatly reduced Ab responses following challenge with human mu-chain in adjuvant. Adoptive transfer experiments showed that the recipient's Th cell response to human mu-chain was impaired. When the human mu transgenic spleen cells were activated with LPS before transfer, they no longer induced tolerance. Spleen cells from double-transgenic mice expressing both human mu-chain and the costimulatory molecule B7-1 (CD80) also failed to induce tolerance to human mu-chain. However, neither human mu transgenic LPS blasts nor double-transgenic B cells induced an Ab response or primed for a secondary Ab response to Ag in adjuvant. Therefore, we find that expression of B7-1 together with Ag can interfere with tolerance induction without inducing Ab formation or priming for a secondary Ab response.
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Citation: J Immunol. 1996 Sep 1;157(5):1987-95.
Journal of immunology (Baltimore, Md. : 1950)
Yuschenkoff, Victoria Nicole; Sethna, Michael P.; Freeman, Gordon J.; and Parker, David C., "Coexpression of B7-1 and antigen blocks tolerance induction to antigen presented by resting B cells" (1996). GSBS Student Publications. 706.