GSBS Student Publications

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Program in Molecular Medicine

Date

9-16-2004

Document Type

Article

Medical Subject Headings

Animals; Biochemistry; Cell Line, Tumor; Cell Nucleus; Chromatin; Computational Biology; Core Binding Factor alpha Subunits; DNA-Binding Proteins; *Gene Expression Regulation; Hela Cells; Humans; Image Processing, Computer-Assisted; Microscopy, Fluorescence; Mitosis; Mutation; Neoplasm Proteins; Nuclear Matrix; Nuclear Proteins; Point Mutation; Protein Structure, Tertiary; Rats; Transcription Factors; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are required to understand the assembly of regulatory machinery within the context of nuclear architecture and to provide a mechanistic link with biological control. We have developed 'intranuclear informatics' to quantify functionally relevant parameters of spatially organized nuclear domains. Using this informatics strategy we have characterized post-mitotic reestablishment of focal subnuclear organization of Runx (AML/Cbfa) transcription factors in progeny cells. By analyzing point mutations that abrogate fidelity of Runx intranuclear targeting, we establish molecular determinants for the spatial order of Runx domains. Our novel approach provides evidence that architectural organization of Runx factors may be fundamental to their tissue-specific regulatory function.

Rights and Permissions

Citation: J Cell Sci. 2004 Oct 1;117(Pt 21):4889-96. Epub 2004 Sep 14. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Journal of cell science

PubMed ID

15367579

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.