GSBS Student Publications

Title

Structural basis for preferential recognition of diaminopimelic acid-type peptidoglycan by a subset of peptidoglycan recognition proteins

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Medicine, Division of Infectious Diseases and Immunology

Date

1-24-2006

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Arginine; Calorimetry; Carrier Proteins; Cell Wall; Crystallography, X-Ray; Diaminopimelic Acid; Dimerization; Drosophila; Escherichia coli; Gram-Negative Bacteria; Ions; Kinetics; Ligands; Lysine; Models, Chemical; Models, Molecular; Molecular Sequence Data; Peptides; Peptidoglycan; Plasmids; Polymers; Prostaglandins; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Signal Transduction; Temperature

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Drosophila peptidoglycan recognition protein (PGRP)-LCx and -LCa are receptors that preferentially recognize meso-diaminopimelic acid (DAP)-type peptidoglycan (PGN) present in Gram-negative bacteria over lysine-type PGN of gram-positive bacteria and initiate the IMD signaling pathway, whereas PGRP-LE plays a synergistic role in this process of innate immune defense. How these receptors can distinguish the two types of PGN remains unclear. Here the structure of the PGRP domain of Drosophila PGRP-LE in complex with tracheal cytotoxin (TCT), the monomeric DAP-type PGN, reveals a buried ionic interaction between the unique carboxyl group of DAP and a previously unrecognized arginine residue. This arginine is conserved in the known DAP-type PGN-interacting PGRPs and contributes significantly to the affinity of the protein for the ligand. Unexpectedly, TCT induces infinite head-to-tail dimerization of PGRP-LE, in which the disaccharide moiety, but not the peptide stem, of TCT is positioned at the dimer interface. A sequence comparison suggests that TCT induces heterodimerization of the ectodomains of PGRP-LCx and -LCa in a closely analogous manner to prime the IMD signaling pathway, except that the heterodimer formation is nonperpetuating.

Rights and Permissions

Citation: J Biol Chem. 2006 Mar 24;281(12):8286-95. Epub 2006 Jan 20. Link to article on publisher's site

Related Resources

Link to article in PubMed

Journal Title

The Journal of biological chemistry

PubMed ID

16428381