Title

Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

8-15-2002

Document Type

Article

Medical Subject Headings

Animals; Bone and Bones; Chondrocytes; Core Binding Factor Alpha 1 Subunit; Mesoderm; Mice; Mice, Transgenic; Models, Genetic; *Neoplasm Proteins; *Promoter Regions (Genetics); Protein Isoforms; Signal Transduction; Time Factors; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.

Rights and Permissions

Citation: Mech Dev. 2002 Jun;114(1-2):167-70.

Related Resources

Link to article in PubMed

PubMed ID

12175505