Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Animals; Bone and Bones; Chondrocytes; Core Binding Factor Alpha 1 Subunit; Mesoderm; Mice; Mice, Transgenic; Models, Genetic; *Neoplasm Proteins; *Promoter Regions (Genetics); Protein Isoforms; Signal Transduction; Time Factors; Transcription Factors
Life Sciences | Medicine and Health Sciences
The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.
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Citation: Mech Dev. 2002 Jun;114(1-2):167-70.