GSBS Student Publications

Title

Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

9-3-1999

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Carrier Proteins; Cloning, Molecular; Fungal Proteins; *Gene Expression Regulation, Fungal; *Genes, Fungal; Genetic Complementation Test; Half-Life; Molecular Sequence Data; Mutation; Polyribosomes; RNA, Fungal; RNA, Messenger; *RNA-Binding Proteins; Ribosomal Proteins; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Suppression, Genetic; Temperature; Threonine-tRNA Ligase

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

In a screen for factors involved in mRNA turnover, four temperature-sensitive yeast strains (ts1189, ts942, ts817, and ts1100) exhibited defects in the decay of several mRNAs. Complementation of the growth and mRNA decay defects, and genetic experiments, revealed that ts1189 is mutated in the previously unknown MRT4 gene, ts942 is mutated in GRC5 (encoding the L9 ribosomal protein), ts817 contains a mutation in SLA2 (encoding a membrane protein), and ts1100 contains a mutation in THS1 (encoding the threonyl-tRNA synthetase). Three of the four mutants (mrt4, grc5, and sla2) were not defective in protein synthesis, suggesting that these strains contain mutations in factors that may play a specific role in mRNA decay. The mRNA stabilization observed in the ths1 strain, however, could be due to the significant drop in translation observed in this mutant at 37 degrees. While the three interesting mutants appear to encode novel mRNA decay factors, at least one could be linked to a previously characterized mRNA decay pathway. The growth and mRNA decay defects of ts942 (grc5) cells were suppressed by overexpression of the NMD3 gene, encoding a protein shown to participate in a two-hybrid interaction with the nonsense-mediated decay protein Upf1p.

Rights and Permissions

Citation: Genetics. 1999 Sep;153(1):35-47.

Related Resources

Link to Article in PubMed

Journal Title

Genetics

PubMed ID

10471698