GSBS Student Publications

Title

Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Molecular Medicine; Program in Gene Function and Expression

Date

1-13-2001

Document Type

Article

Medical Subject Headings

Adenosine Triphosphatases; Amino Acid Sequence; Cloning, Molecular; Conserved Sequence; Fungal Proteins; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; *Nuclear Proteins; *RNA Splicing; Recombinant Proteins; Ribonucleoproteins; Saccharomyces cerevisiae; Sequence Alignment; Sequence Homology, Amino Acid

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The human 56-kD U2AF(65)-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.

Rights and Permissions

Citation: Genes Dev. 2001 Jan 1;15(1):30-5.

Related Resources

Link to Article in PubMed

Journal Title

Genes and development

PubMed ID

11156602