The homeodomain transcription factor CDP/cut interacts with the cell cycle regulatory element of histone H4 genes packaged into nucleosomes
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Amino Acid Motifs; Binding Sites; Cell Cycle Proteins; Chromatin; DNA; DNA Footprinting; DNA-Binding Proteins; Genes, cdc; Hela Cells; Histones; Homeodomain Proteins; Humans; Nuclear Proteins; Nucleosomes; Promoter Regions (Genetics); Protein Binding; Recombinant Proteins; Repressor Proteins; Transcription Factors
Life Sciences | Medicine and Health Sciences
The homeodomain transcription factor CDP/cut contains four separate DNA binding domains and interacts with large segments of DNA. Thus, CDP/cut has the potential to function as an architectural protein and perhaps to support modifications in chromatin structure and nucleosomal organization. To begin to examine the ability of CDP/cut to interact with chromatin, we analyzed binding of CDP/cut to the histone H4 gene promoter (-90 to +75) reconstituted into nucleosome cores. The -90 to +75 region encompasses the cell cycle regulatory element (Site II) that controls histone H4 gene transcription, a CDP/cut binding site and a nuclease hypersensitive region. Using electrophoretic mobility shift assays and DNase I footprinting experiments, we show that CDP/cut specifically interacts with its recognition motif in a nucleosomal context without displacing the nucleosome core. The competency of CDP/cut to interact with nucleosomes suggests that this transcription factor may facilitate chromatin remodeling in response to cell cycle regulatory and/or developmental cues.
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Citation: Mol Biol Rep. 1999 Aug;26(3):185-94.