GSBS Student Publications

Title

Construction of cloned libraries from RNA of human fetal tissues

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

1-1-1982

Document Type

Article

Medical Subject Headings

*Cloning, Molecular; DNA; DNA, Recombinant; Escherichia coli; Female; *Fetus; Humans; Plasmids; Poly A; Pregnancy; RNA

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.

Rights and Permissions

Citation: Cytogenet Cell Genet. 1982;34(3):193-203.

Related Resources

Link to article in PubMed

Journal Title

Cytogenetics and cell genetics

PubMed ID

6183056