The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Pathology
Medical Subject Headings
Animals; Animals, Newborn; Antigens, CD44; CD8-Positive T-Lymphocytes; *Cell Differentiation; Cell Proliferation; Cells, Cultured; Immunologic Memory; Interleukin-15; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Mice, Knockout; Phenotype; Protein-Tyrosine Kinases; Receptors, Antigen, T-Cell; T-Box Domain Proteins; Thymus Gland; Time Factors; Up-Regulation; Xenopus Proteins
Life Sciences | Medicine and Health Sciences
The Tec family tyrosine kinases, Itk and Rlk, are expressed in thymocytes and peripheral T cells and regulate thresholds of T cell receptor signaling. Yet little is known about the specific role of Itk- and Rlk-dependent signals in CD8(+) T cell maturation. We show here that Itk(-/-) and Rlk(-/-)Itk(-/-) mice were nearly devoid of conventional CD8(+) T cells and, instead, contained a large population of CD8(+) T cells that bear striking similarity to lineages of innate lymphocytes. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes and T cells were CD44(hi), CD122(+), and NK1.1(+); were able to produce interferon-gamma directly ex vivo; and were dependent on interleukin-15. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes expressed abundant transcripts for the T box transcription factor, eomesodermin, correlating with their phenotype and function. These data indicate a critical role for Itk and Rlk in conventional CD8(+) T cell development in the thymus.
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Citation: Immunity. 2006 Jul;25(1):79-91. Link to article on publisher's site
Atherly, Luana O.; Lucas, Julie Ann; Felices, Martin; Yin, Catherine C.; Reiner, Steven L.; and Berg, Leslie J., "The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells" (2006). GSBS Student Publications. Paper 63.