GSBS Student Publications

Title

Antibody-dependent cell-mediated cytotoxicity directed by a human monoclonal antibody reactive with gp120 of HIV-1

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pediatrics

Date

11-1-1991

Document Type

Article

Medical Subject Headings

*Antibodies, Monoclonal; *Antibody-Dependent Cell Cytotoxicity; Binding Sites; Cell Line; Epitopes; Gene Products, env; *HIV Antibodies; HIV Envelope Protein gp120; HIV Envelope Protein gp160; HIV-1; Humans; Protein Precursors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We used a human monoclonal antibody (MAb; 15e) to identify an antibody-dependent cell-mediated cytotoxicity (ADCC) epitope on HIV-1 gp120. 15e has been shown to recognize a conformation-dependent epitope on gp120 which is important in both CD4 binding and neutralizing of HIV-1 infection. 15e binds to gp120 of HIV-1IIIB but not HIV-1RF. Using a standard ADCC assay, 15e was found to mediate ADCC against cells infected with HIV-1IIIB but not HIV-1RF. 15e did not mediate ADCC against cells with recombinant gp120 bound to surface CD4, indicating that 15e does not mediate innocent bystander ADCC against uninfected CD4 cells. To better define the 15e epitope, we performed ADCC against target cells infected with a vaccinia vector which expresses processed HIV-1IIIB gp160 from which the third variable region was deleted (amino acids, 312-328). MAb 15e efficiently mediated ADCC against cells expressing this altered form of gp120, indicating that this region is not contributing to the conformational epitope defined by 15e. 15e defines an important epitope in the human immune response to HIV-1 infection. Antibodies with 15e-like activity may be useful in immunoprophylaxis or immunotherapy of HIV-1 infection.

Rights and Permissions

Citation: AIDS. 1991 Nov;5(11):1309-14.

Related Resources

Link to article in PubMed

Journal Title

AIDS (London, England)

PubMed ID

1722676