GSBS Student Publications

Title

Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

10-1-1987

Document Type

Article

Medical Subject Headings

Alleles; DNA, Viral; Gene Expression Regulation; *Genes, Regulator; *Genes, Viral; Muramidase; Mutation; Plasmids; Suppression, Genetic; T-Phages; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream from the lysozyme gene, was constructed. Amber mutations were introduced into gene e in the hybrid phage, and the resulting mutant phages were tested for the ability to form plaques on amber suppressor strains. Revertant phages that were able to form plaques on amber suppressors that did not suppress the parent amber mutant phages were isolated following UV mutagenesis. Secondary site pseudorevertants were identified among the revertants by a genetic test. Four of the suppressing secondary site mutations were mapped and sequenced. They were found to consist of small sequence alterations immediately upstream from gene e, all of which would tend to destabilize potential base-pairing interactions in the transcript. The mutations were shown to increase lysozyme expression when introduced into an otherwise wild-type hybrid phage, but were found to have little effect on transcription of the lysozyme gene.

Rights and Permissions

Citation: J Bacteriol. 1987 Oct;169(10):4630-6.

Related Resources

Link to article in PubMed

Journal Title

Journal of bacteriology

PubMed ID

3654580