GSBS Student Publications

Title

The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

11-30-2005

Document Type

Article

Medical Subject Headings

Animals; Antigens, Differentiation; Cell Differentiation; *Cell Proliferation; Core Binding Factor Alpha 1 Subunit; Gene Expression Regulation; Mesoderm; Mice; NIH 3T3 Cells; Osteoblasts; Osteogenesis; Signal Transduction; Transcription Factors; Transfection

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Osterix is a bone-related transcription factor that functions genetically downstream of Runx2, which controls both growth and differentiation in osteoblasts. Here we assessed the biological function of Osterix in mesenchymal cells that are not normally committed to the osteogenic lineage. Stably transfected NIH3T3 fibroblasts that express exogenous Osterix were examined for their ability to convert into osteoblastic cells by analyzing gene expression profiles of bone phenotype related markers, as well as by measuring bone nodule formation and cell proliferation. Forced expression of Osterix stimulates osteopontin gene expression but not the expression or activity of other bone-related markers, including collagen type I, alkaline phosphatase, osteocalcin, or osteonectin. Moreover, cells stably expressing Osterix do not induce bone nodule formation. Strikingly, both polyclonal and monoclonal cells expressing Osterix exhibit enhanced proliferation. Collectively, these results indicate that Osterix is insufficient to establish osteogenic lineage commitment, perhaps due to the ability of Osterix to promote cell growth. We propose that regulatory pathways operating upstream of or in parallel with Osterix are required for osteogenic conversion of uncommitted mesenchymal cells.

Rights and Permissions

Citation: Gene. 2006 Jan 17;366(1):145-51. Epub 2005 Nov 28. Link to article on publisher's site

DOI of Published Version

10.1016/j.gene.2005.08.021

Related Resources

Link to article in PubMed

Journal Title

Gene

PubMed ID

16314050