GSBS Student Publications

Title

Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Medicine; Department of Cell Biology

Date

9-1-2004

Document Type

Article

Medical Subject Headings

Animals; Antigens; Carrier Proteins; Cell Movement; Cells, Cultured; Cilia; Epithelial Cells; Immunohistochemistry; Macromolecular Substances; Male; Membrane Proteins; Mice; Microscopy, Electron; Protein Transport; RNA Interference; Spermatocytes; TRPP Cation Channels

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Primary cilia are nonmotile microtubule structures that assemble from basal bodies by a process called intraflagellar transport (IFT) and are associated with several human diseases. Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and motile cilia. Immunogold electron microscopy demonstrates that Pcnt is on or near basal bodies at the base of cilia. Pcnt depletion by RNA interference disrupts basal body localization of IFT proteins and the cation channel polycystin-2 (PC2), and inhibits primary cilia assembly in human epithelial cells. Conversely, silencing of IFT20 mislocalizes Pcnt from basal bodies and inhibits primary cilia assembly. Pcnt is found in spermatocyte IFT fractions, and IFT proteins are found in isolated centrosome fractions. Pcnt antibodies coimmunoprecipitate IFT proteins and PC2 from several cell lines and tissues. We conclude that Pcnt, IFTs, and PC2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella.

Rights and Permissions

Citation: J Cell Biol. 2004 Aug 30;166(5):637-43. Link to article on publisher's site

Related Resources

Link to article in PubMed

Journal Title

The Journal of cell biology

PubMed ID

15337773