Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly
Authors
Jurczyk, AgataGromley, Adam Scott
Redick, Sambra D.
San Agustin, Jovenal T.
Witman, George B.
Pazour, Gregory J.
Peters, Dorien J. M.
Doxsey, Stephen J.
UMass Chan Affiliations
Department of Cell BiologyDepartment of Molecular Medicine
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2004-09-01Keywords
Animals; Antigens; Carrier Proteins; Cell Movement; Cells, Cultured; Cilia; Epithelial Cells; Immunohistochemistry; Macromolecular Substances; Male; Membrane Proteins; Mice; Microscopy, Electron; Protein Transport; RNA Interference; Spermatocytes; TRPP Cation ChannelsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Primary cilia are nonmotile microtubule structures that assemble from basal bodies by a process called intraflagellar transport (IFT) and are associated with several human diseases. Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and motile cilia. Immunogold electron microscopy demonstrates that Pcnt is on or near basal bodies at the base of cilia. Pcnt depletion by RNA interference disrupts basal body localization of IFT proteins and the cation channel polycystin-2 (PC2), and inhibits primary cilia assembly in human epithelial cells. Conversely, silencing of IFT20 mislocalizes Pcnt from basal bodies and inhibits primary cilia assembly. Pcnt is found in spermatocyte IFT fractions, and IFT proteins are found in isolated centrosome fractions. Pcnt antibodies coimmunoprecipitate IFT proteins and PC2 from several cell lines and tissues. We conclude that Pcnt, IFTs, and PC2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella.Source
J Cell Biol. 2004 Aug 30;166(5):637-43. Link to article on publisher's siteDOI
10.1083/jcb.200405023Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33935PubMed ID
15337773Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1083/jcb.200405023