GSBS Student Publications

Title

Mutant and native human beta-amyloid precursor proteins in transgenic mouse brain

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

7-1-1995

Document Type

Article

Medical Subject Headings

Amyloid beta-Protein Precursor; Animals; Brain; Female; Humans; Immunoblotting; In Situ Hybridization; Mice; Mice, Transgenic; *Mutation; Precipitin Tests; Promoter Regions (Genetics); RNA; Synapsins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Human beta-amyloid precursor protein (beta APP) has been targeted to transgenic neurons using synapsin I promoter-based chimeric transgenes. Native human beta APP was introduced as well as beta APP containing mutations genetically linked to familial Alzheimer's disease (AD) and to hereditary cerebral hemorrhage with amyloidosis-Dutch type. In mouse brain, human beta APP RNA was up to 60% as abundant as total endogenous beta APP RNA. Human beta APP gene expression was most abundant in the CA subfields of the hippocampus and in the piriform cortex. Correct processing of human beta APP at the beta-secretase cleavage site was demonstrated in transgenic mouse brains. Despite a 40% increase in total beta APP immunoreactivity in lines expressing mutant human beta APP, no evidence of amyloid deposition was found in brains of mice up to 14 months in age. Higher levels of mutant human beta APP, increased age, or other factors may be necessary to elicit beta-amyloid-related neuropathologies in the rodent brain.

Rights and Permissions

Citation: Neurobiol Aging. 1995 Jul-Aug;16(4):685-99.

Related Resources

Link to article in PubMed

Journal Title

Neurobiology of aging

PubMed ID

8544921