Mutant and native human beta-amyloid precursor proteins in transgenic mouse brain
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Amyloid beta-Protein Precursor; Animals; Brain; Female; Humans; Immunoblotting; In Situ Hybridization; Mice; Mice, Transgenic; *Mutation; Precipitin Tests; Promoter Regions (Genetics); RNA; Synapsins
Life Sciences | Medicine and Health Sciences
Human beta-amyloid precursor protein (beta APP) has been targeted to transgenic neurons using synapsin I promoter-based chimeric transgenes. Native human beta APP was introduced as well as beta APP containing mutations genetically linked to familial Alzheimer's disease (AD) and to hereditary cerebral hemorrhage with amyloidosis-Dutch type. In mouse brain, human beta APP RNA was up to 60% as abundant as total endogenous beta APP RNA. Human beta APP gene expression was most abundant in the CA subfields of the hippocampus and in the piriform cortex. Correct processing of human beta APP at the beta-secretase cleavage site was demonstrated in transgenic mouse brains. Despite a 40% increase in total beta APP immunoreactivity in lines expressing mutant human beta APP, no evidence of amyloid deposition was found in brains of mice up to 14 months in age. Higher levels of mutant human beta APP, increased age, or other factors may be necessary to elicit beta-amyloid-related neuropathologies in the rodent brain.
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Citation: Neurobiol Aging. 1995 Jul-Aug;16(4):685-99.
Neurobiology of aging
Howland, David S.; Savage, Mary J.; Huntress, Frederick A.; Wallace, Racheal E.; Schwartz, Daniel A.; Loh, Tamalette; Melloni, Richard H.; DeGennaro, Louis J.; Greenberg, Barry D.; and Siman, Robert, "Mutant and native human beta-amyloid precursor proteins in transgenic mouse brain" (1995). GSBS Student Publications. 551.