GSBS Student Publications

Title

Fabrication of tissue engineered tympanic membrane patches using computer-aided design and injection molding

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Center for Tissue Engineering; Department of Otolaryngology--Head and Neck Surgery

Date

7-6-2004

Document Type

Article

Medical Subject Headings

Analysis of Variance; Animals; *Cartilage; Cattle; Chondrocytes; *Computer-Aided Design; Tissue Engineering; *Tympanic Membrane

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

OBJECTIVES/HYPOTHESIS: The goal of the current study was to use computer-aided design and injection molding technologies to tissue engineer precisely shaped cartilage in the shape of butterfly tympanic membrane patches out of chondrocyte-seeded calcium alginate gels. METHODS: Molds were designed on SolidWorks 2000 and built out of acrylonitrile butadiene styrene (ABS) using fused deposition modeling (FDM). Tympanic membrane patches were fabricated using bovine articular chondrocytes seeded at 50 x 10 cells/mL in 2% calcium alginate gels. Molded patches were cultured in vitro for up to 10 weeks and assessed biochemically, morphologically, and histologically. RESULTS: Unmolded patches demonstrated outstanding dimensional fidelity, with a volumetric precision of at least 3 microL, and maintained their shape well for up to 10 weeks of in vitro culture. Glycosaminoglycan and collagen content increased steadily over 10 weeks in culture, demonstrating continual deposition of new extracellular matrix consistent with new tissue development. CONCLUSIONS: The use of computer-aided design and injection molding technologies allows for the fabrication of very small, precisely shaped chondrocyte-seeded calcium alginate structures that faithfully maintain their shape during in vitro culture. In vitro fabrication of tympanic membrane patches with a precisely controlled geometry may have the potential to provide a minimally invasive alternative to traditional methods for the repair of chronic tympanic membrane perforations.

Rights and Permissions

Citation: Laryngoscope. 2004 Jul;114(7):1290-5.

Related Resources

Link to article in PubMed

Journal Title

The Laryngoscope

PubMed ID

15235363