Title

The core of the motor domain determines the direction of myosin movement

GSBS Program

Biochemistry & Molecular Pharmacology

Date

8-24-2001

Document Type

Article

Medical Subject Headings

Animals; Calmodulin; Cell Line; Mice; Molecular Motor Proteins; Movement; Myosins; Protein Structure, Tertiary; Rabbits; Recombinant Fusion Proteins; Structure-Activity Relationship; Xenopus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Myosins constitute a superfamily of at least 18 known classes of molecular motors that move along actin filaments. Myosins move towards the plus end of F-actin filaments; however, it was shown recently that a certain class of myosin, class VI myosin, moves towards the opposite end of F-actin, that is, in the minus direction. As there is a large, unique insertion in the myosin VI head domain between the motor domain and the light-chain-binding domain (the lever arm), it was thought that this insertion alters the angle of the lever-arm switch movement, thereby changing the direction of motility. Here we determine the direction of motility of chimaeric myosins that comprise the motor domain and the lever-arm domain (containing an insert) from myosins that have movement in the opposite direction. The results show that the motor core domain, but neither the large insert nor the converter domain, determines the direction of myosin motility.

Rights and Permissions

Citation: Nature. 2001 Aug 23;412(6849):831-4. Link to article on publisher's site

Related Resources

Link to article in PubMed