Department of Cell Biology
Medical Subject Headings
Bromodeoxyuridine; Calcium-Binding Proteins; Cell Cycle; Cells, Cultured; Centrioles; Centrosome; Chromosomal Proteins, Non-Histone; Enzyme Inhibitors; Epithelial Cells; G1 Phase; Humans; Imidazoles; Light; Microscopy, Electron; Pyridines; p38 Mitogen-Activated Protein Kinases
Cell Biology | Life Sciences | Medicine and Health Sciences
How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
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Citation: J Cell Biol. 2007 Jan 15;176(2):173-82. Link to article on publisher's site