Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells
Authors
Uetake, YumiLoncarek, Jadranka
Nordberg, Joshua J.
English, Christopher N.
La Terra, Sabrina
Khodjakov, Alexey
Sluder, Greenfield
Student Authors
Joshua J. NordbergUMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2007-01-18Keywords
Bromodeoxyuridine; Calcium-Binding Proteins; Cell Cycle; Cells, Cultured; Centrioles; Centrosome; Chromosomal Proteins, Non-Histone; Enzyme Inhibitors; Epithelial Cells; G1 Phase; Humans; Imidazoles; Light; Microscopy, Electron; Pyridines; p38 Mitogen-Activated Protein KinasesCell Biology
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.Source
J Cell Biol. 2007 Jan 15;176(2):173-82. Link to article on publisher's siteDOI
10.1083/jcb.200607073Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33857PubMed ID
17227892Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1083/jcb.200607073