Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pediatrics
Medical Subject Headings
Age Factors; Animals; Antigens, CD3; Antigens, CD4; Antigens, CD8; Blood Cells; Humans; Immune System; Immunoglobulins; Leukocytes, Mononuclear; Mice; Mice, SCID; Models, Biological; Peritoneal Cavity; Phenotype; Receptors, Interleukin-2; Species Specificity; Specific Pathogen-Free Organisms; Spleen; Time Factors; Transplantation, Heterologous
Life Sciences | Medicine and Health Sciences
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.
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Citation: J Infect Dis. 1993 Sep;168(3):630-40.
The Journal of infectious diseases
Hesselton, RuthAnn M.; Koup, Richard A.; Cromwell, Mary A.; Graham, Barney S.; Johns, Michael; and Sullivan, John L., "Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution" (1993). GSBS Student Publications. 493.