GSBS Student Publications

Title

The high resolution crystal structure of deoxyhemoglobin S

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

11-5-1997

Document Type

Article

Medical Subject Headings

*Anemia, Sickle Cell; Computer Simulation; Hemoglobin, Sickle; Humans; Models, Molecular; Molecular Sequence Data; Movement; Polymers; Protein Conformation

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have refined the crystal structure of deoxyhemoglobin S (beta Glu6-->Val) at 2.05 A resolution to an R-factor of 16.5% (free R=21. 5%) using crystals isomorphous to those originally grown by Wishner and Love. A predominant feature of this crystal form is a double strand of hemoglobin tetramers that has been shown by a variety of techniques to be the fundamental building block of the intracellular sickle cell fiber. The double strand is stabilized by lateral contacts involving the mutant valine interacting with a pocket between the E and F helices on another tetramer. The new structure reveals some marked differences from the previously refined 3.0 A resolution structure, including several residues in the lateral contact which have shifted by as much as 3.5 A. The lateral contact includes, in addition to the hydrophobic interactions involving the mutant valine, hydrophilic interactions and bridging water molecules at the periphery of the contact. This structure provides further insights into hemoglobin polymerization and may be useful for the structure-based design of therapeutic agents to treat sickle cell disease.

Rights and Permissions

Citation: J Mol Biol. 1997 Sep 26;272(3):398-407. Link to article on publisher's site

DOI of Published Version

10.1006/jmbi.1997.1253

Related Resources

Link to article in PubMed

Journal Title

Journal of molecular biology

PubMed ID

9325099