GSBS Student Publications

Title

Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology

Date

7-20-1999

Document Type

Article

Medical Subject Headings

Animals; Drosophila; Gene Expression Regulation; Genes, Insect; Immunity; NF-kappa B; Oncogene Proteins v-rel; Retroviridae Proteins, Oncogenic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

NF-kappaB/Rel family proteins regulate genes that are critical for many cellular processes including apoptosis, inflammation, immune response, and development. NF-kappaB/Rel proteins function as homodimers or heterodimers, which recognize specific DNA sequences within target promoters. We examined the activity of different Drosophila Rel-related proteins in modulating Drosophila immunity genes by expressing the Rel proteins in stably transfected cell lines. We also compared how different combinations of these transcriptional regulators control the activity of various immunity genes. The results show that Rel proteins are directly involved in regulating the Drosophila antimicrobial response. Furthermore, the drosomycin and defensin expression is best induced by the Relish/Dif and the Relish/Dorsal heterodimers, respectively, whereas the attacin activity can be efficiently up-regulated by the Relish homodimer and heterodimers. These results illustrate how the formation of Rel protein dimers differentially regulate target gene expression.

Rights and Permissions

Citation: J Biol Chem. 1999 Jul 23;274(30):21355-61.

Related Resources

Link to article in PubMed

Journal Title

The Journal of biological chemistry

PubMed ID

10409696